| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Christensen, Kate L (12)
|
IADSMY |
35.81 |
34.49 |
-1.32 |
| 2 |
Haltom, Kelsey (12)
|
SPS |
37.12 |
35.82 |
-1.30 |
| 3 |
Witt, RyAnn (12)
|
SPS |
36.40 |
36.20 |
-0.20 |
| 4 |
Cline, Haven A (11)
|
IADSMY |
39.49 |
39.54 |
0.05 |
| 5 |
Hoffman, Kelly L (12)
|
IADSMY |
37.50 |
39.61 |
2.11 |
| 6 |
Oiler, Allison V (12)
|
IADSMY |
39.22 |
40.18 |
0.96 |
| 7 |
Klein, Addison G (12)
|
IADSMY |
39.72 |
40.70 |
0.98 |
| 8 |
Holt, Anne A (12)
|
IADSMY |
45.25 |
42.99 |
-2.26 |
| 9 |
Strunk, Mia (12)
|
SPS |
50.74 |
45.32 |
-5.42 |
| 10 |
Nanaziashvili, Sasha (11)
|
IADSMY |
48.71 |
47.30 |
-1.41 |
| 11 |
van den Berge, Marley J (11)
|
IADSMY |
|
48.39 |
- |
| 12 |
Vance, Allana C (12)
|
IADSMY |
51.05 |
54.85 |
3.80 |
| 13 |
Guiter, Reagan (11)
|
SPS |
1:08.76 |
1:06.23 |
-2.53 |
|
Ellis, Ryann I (12)
|
IAMACR |
|
DQ |
- |
|
Smith, Isabella A (11)
|
IADSMY |
44.84 |
NS |
- |